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| Registros recuperados: 66 | |
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| Guimarães,Simone Eliza Facioni; Euclydes,Ricardo Frederico. |
| O objetivo deste trabalho foi determinar a freqüência do gene causador da síndrome do estresse em suíno (PSS) e sua associação com características reprodutivas em marrãs híbridas. Setenta e duas marrãs híbridas Landrace x Large White, provenientes de granjas comerciais localizadas na região de Ponte Nova - Minas Gerais, foram analisadas para a presença da mutação PSS. No diagnóstico da mutação feito por reação em cadeia da polimerase - polimorfismo de comprimento de fragmentos de restrição (PCR-RFLP), o fragmento de 659pb do gene ryr-1 foi amplificado e, nos mutantes a substituição de citosina por timina, que acarreta mudança de aminoácidos da proteína madura de arginina para cisteína nos animais afetados, foi identificado. Dados relativos ao número de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PCR-RFLP; Marrãs híbridas; PSS. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35981999000300008 |
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| Gomes,Eliane A.; Kasuya,Maria Catarina M.; Barros,Everaldo G. de; Borges,Arnaldo C.; Araújo,Elza F.. |
| Inter- and intraspecific variation among 26 isolates of ectomycorrhizal fungi belonging to 8 genera and 19 species were evaluated by analysis of the internal transcribed sequence (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplification products, which ranged between 560 and 750 base pairs (bp), were obtained for all the isolates analyzed. The degree of polymorphism observed did not allow proper identification of most of the isolates. Cleavage of amplified fragments with the restriction enzymes Alu I, Hae III, Hinf I, and Hpa II revealed extensive polymorphism. All eight genera... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA fingerprinting; Ectomycorrhizal fungi; Internal transcribed spacer (ITS); PCR-RFLP; Ribosomal DNA (rDNA). |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400018 |
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| Faure, Stephanie. |
| La marteiliose est une maladie causée par Marteilia refringens chez l'huître plate, Ostrea edulis. Ce parasite induit des mortalités importantes chez ces mollusques bivalves. Une autre espèce, Marteilia maurini, parasite la moule, Mytilus edulis, sans engendrer de mortalités notables. Des cas de coinfections des moules par ces 2 parasites ont été observés. La moule pourrait donc être vecteur de Marteilia refringens. Des études sur la taxinomie de Marteilia refringens ont démontré qu'il existe un polymorphisme entre les 2 espèces dans l'ITS1. De ce fait, une technique de PCR-RFLP a été mise au point pour discriminer les 2 espèces. Le but de cette étude est d'éclaircir le statut de la moule dans le cycle biologique de Marteilia refringens par deux approches... |
| Tipo: Text |
Palavras-chave: Marteilia refringens; Marteilia maurini; Mytilus edulis; Vecteur; PCR-RFLP; PCR; Pathologie. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00032/14343/11627.pdf |
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| WEIKERT-OLIVEIRA,RITA C. B.; RESENDE,M. APARECIDA DE; VALÉRIO,HENRIQUE M.; CALIGIORNE,RACHEL B.; PAIVA,EDILSON. |
| Twenty isolates of four fungal species, agents of "Helminthosporium" diseases in cereals, were collected from different regions: nine Bipolarisoryzae isolated from rice (Oryza sativa), seven B.sorokiniana from wheat (Triticum aestivum), two B. maydis, and two Exserohilumturcicum from maize (Zea mays). The strains were compared by PCR-RFLP and RAPD analysis. Size polymorphism among the isolates in the ITS region comprising the 5.8 S rDNA indicated genetic differences among the isolates, while a UPGMA phenogram constructed after the digestion of this region with restriction enzymes showed inter- and intra-specific polymorphism. The RAPD profiles indicated an expressive level of polymorphism among different species, compared with a low level of polymorphism... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bipolaris sorokiniana; Bipolaris oryzae; Bipolaris maydis; Exserohilum turcicum; PCR-RFLP; RAPD. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000600015 |
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| Mathia, Olivier. |
| Marteilia refringens est l'un des parasites de l'huître plate, Ostrea edulis ; la marteiliose dont il est l'agent causal provoque une forte mortalité chez les mollusques bivalves. Le genre Marteilia comprend d'autres espèces telle que Marteilia maurini qui lui affecte la moule, Mytilus edulis. Il a été démontré, dans quelques rares cas que les deux espèces pouvaient co habiter au sein d'une même moule. Ainsi le mollusque pourrait être vecteur de Marteilia refringens. Certaines études ont montré par PCR et PCR-RFLP qu'il existait un dimorphisme dans la région ITS-1 des gènes ribosomaux chez Marteilia. Ces techniques m'ont donc permis d'observer la prévalence du phénomène de co-detection dans le milieu naturel et d'étudier l'implication de la moule dans le... |
| Tipo: Text |
Palavras-chave: Marteilia refringens; Marteilia maurini; Mytilus edulis; Vecteur; PCR; PCR-RFLP. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00369/47981/48007.pdf |
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| Almeida,Álvaro M. R.; Abdelnoor,Ricardo V.; Arias,Carlos A. Arrabal; Carvalho,Valdemar P.; Jacoud Filho,David S.; Marin,Silvana R. R.; Benato,Luís C.; Pinto,Mauro C.; Carvalho,Cláudio G. P.. |
| Macrophomina phaseolina has been considered one of the most prevalent soybean (Glycine max) pathogens in Brazil. No genetic resistance has been determined in soybean and very little is known about the genetic diversity of this pathogen in tropical and sub-tropical regions. Fifty-five isolates from soybean roots were collected in different regions and analyzed through RAPD for genetic diversity. The UPGMA cluster analysis for 74 loci scored permitted identification of three divergent groups with an average similarity of 99%, 92% and 88%, respectively. The three groups corresponded to 5.45%, 59.95% and 34.6%, respectively of all isolates used. A single plant had three different haplotypes, while 10.9% of the analyzed plants had two different haplotypes. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAPD; ITS; PCR-RFLP; Soybean charcoal rot. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000300009 |
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| Destéfano,Ricardo Henri Rodrigues; Destéfano,Suzete A. Lanza; Messias,Claudio Luiz. |
| In order to construct specific primers for the detection and identification of the entomopathogenic fungus Metarhizium within infected sugarcane borer (Diatraea saccharalis) larvae we analyzed the ITS1 -5.8S- ITS2 rDNA regions of strains and varieties of M. anisopliae, M. album and M. flavoviride. The PCR amplification of these regions yielded a unique fragment of approximately 540 bp for M. anisopliae variety anisopliae strains E9, B/Vi and C (isolated in Brazil), 600 pb for M. a. anisopliae strain 14 (isolated in Australia), 650 bp for the M. album and 600 bp for M. flavoviride strains. The PCR products were digested with different restriction endonucleases (Afa I, Alu I, Dde I, Hae III, Hpa II and Sau 3A) and the PCR-RFLP profiles showed clear... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metarhizium anisopliae; Entomopathogenic fungi; PCR-RFLP; ITS region. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200020 |
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| Gibotti,Adriana; Tanaka,Tânia L.; Oliveira,Valéria R.; Taddei,Carla R.; Martinez,Marina B.. |
| In this study, polymorphism in ipa genes was found in five out of nine EIEC serotypes studied. When SalI and HindII were used in RFLP-PCR assays many EIEC serotypes showed polymorphism in ipaB and ipaD. On the other hand, no polymorphism was observed in ipaA and ipaC in these strains. The polymorphism present in EIEC strains is serotype-dependent, since restriction patterns were conserved amongst strains belonging to the same serotype. When IpaB deduced amino acid sequences of S. flexneri M90T and FBC124-13 were compared, ten amino acids changes could be observed mainly in the amino-terminal region. The deduced EIEC IpaD amino-acid sequence presented 91% similarity with the Shigella strain. In this case, amino acid changes were spread out through the whole... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ipa; Enteroinvasive Escherichia coli; Polymorphism; PCR-RFLP. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100012 |
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| Band,Guilherme de Oliveira; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Schierholt,Alex Sandro; Silva,Kleibe Moraes; Pires,Aldrin Vieira; Benevenuto Júnior,Augusto Aloísio; Gomide,Lúcio Alberto de Miranda. |
| The PSS genotypes of 596 F2 pigs produced by initial mating of Brazilian commercial sows and native boars were characterized by PCR-RFLP and the pork quality traits were evaluated. Among the 596 pigs studied, 493 (82.7%) were NN and 103 (17.3%) were Nn. There were no differences between NN and Nn pigs in the following pork qualities: pHu (5.71 ± 0.16 vs 5.70 ± 0.11), intramuscular fat (1.55 ± 0.64% vs 1.65 ± 0.67%), shear force (5552 ± 878 g/1.2 cm vs 5507 ± 826 g/1.2 cm), lightness (44.96 ± 2.05 vs 45.01 ± 1.92), redness (0.64 ± 0.60 vs 0.79 ± 0.55), yellowness (6.62 ± 0.56 vs 6.65 ± 0.48), hue (84.28 ± 5.53 vs 83.41 ± 4.85), or chroma (6.68 ± 0.52 vs 6.73 ± 0.52). However, pork from Nn pigs had a significantly (p < 0.05) lower pH45 (6.41 ± 0.27 vs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Meat quality; PCR-RFLP; Pork; PSE; PSS; Pig. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100015 |
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| Otaviano,Antonio Roberto; Tonhati,Humberto; Sena,Janete Aparecida Desidério; Cerón Muñoz,Mario Fernando. |
| Caseins comprise make up about 80% of the total protein content of milk and present polymorphism with changes in the amino acid sequence. Within this abundance of proteins, kappa-casein is noteworthy, since it has been associated with differences in milk yield, composition and processing. The objective of this study was to observe the existence of polymorphism in the kappa-casein gene in female buffaloes. For this purpose, blood samples from 115 female buffaloes, collected with vacutainer by needle punctionure of the jugular vein, were used. for genomic DNA extraction was done from blood samples. The PCR-RFLP and SSCP techniques demonstrated that the studied animals were monomorphic for the kappa-casein gene. Only allele B was observed in these animals,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Buffaloes; Polymorphism; PCR-RFLP; SSCP. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200010 |
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| Band,Guilherme de Oliveira; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Peixoto,Jane de Oliveira; Faria,Danielle Assis; Pires,Aldrin Vieira; Figueiredo,Frederico de Castro; Nascimento,Carlos Souza do; Gomide,Lúcio Alberto de Miranda. |
| The PSS genotypes of 596 F2 pigs produced by initial mating of Brazilian native boars commercial sows and were characterized by PCR-RFLP and their carcass and performance traits were evaluated. Among the 596 animals analyzed, 493 (82.72%) were characterized as NN and 103 (17.28%) as Nn. With respect to carcass traits, Nn animals presented higher (p < 0.05) right half carcass weight, left half carcass weight, loin depth and loin eye area, and lower shoulder backfat thickness, backfat thickness between last and next to last but one lumbar vertebrae and backfat thickness after last rib at 6.5 cm from the midline compared to NN animals. Nn animals also showed (p < 0.05) higher values for most of the cut yields, indicating higher cutting yields for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carcass; Performance; PSS gene; PCR-RFLP; Pig. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100016 |
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| Choudhary,Vivek; Kumar,Pushpendra; Bhattacharya,Tarun K.; Bhushan,Bharat; Sharma,Arjava. |
| Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle. We used the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique to screen for DNA polymorphisms of the leptin gene in 403 cattle belonging to various breeds of Bos indicus (Hariana, Sahiwal, Gir and Nimari cattle), Bos taurus (Holstein Friesian (HF) and Jersey cattle) as well as Bos taurus x Bos indicus crossbreds (½ HF x ½ Hariana). In all the cattle we amplified two regions of the leptin gene, a 522 bp fragment comprising the partial intron 2 and exon 3 and another 94 bp fragment consisting of part of exon 2. Digestion of 522 bp PCR products with the BsaAI restriction enzyme revealed three genotypes in... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Leptin gene; PCR-RFLP; Polymorphism; Bos indicus; Bos taurus. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500014 |
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| Jaffres, Emmanuel. |
| Les bactéries du genre Vibrio représentent le groupe le plus fréquemment associé à des maladies infectieuses chez les mollusques marins. Ainsi chez l'huître creuse Crassostrea gigas, la majorité des Vibrios isolés d'animaux sains ou malades sont phénotypiquement classés dans un groupe de souches apparentées à Vibrio splendidus. Les récentes études phylogénétiques pars séquençage du gène gyrB, ont permis de distinguer plusieurs espèces au sein de ce groupe (V. splendidus, V. chagasii, V. cyclitrophicus, V. crassostreae, V. gigantis). Toutefois, il subsiste toujours des imprécisions dans l'arbre phylogénétique gyrB. En effet, un groupe polyphylétique de 4 espèces reste jusqu'à présent on distinguables (V. tasmaniensis, V. kanaloae, V. pomeroyi, V. lentus).... |
| Tipo: Text |
Palavras-chave: Mortalités estivales; Huître creuse; Crassostrea gigas; Vibrio spendidus; PCR-RFLP; Séquençage; Gène ADNr-16S; GyrB; Macroarrays. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00034/14479/11779.pdf |
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| Santos,MMAB; Peiró,JR; Meireles,MV. |
| Avian cryptosporidiosis has been reported in more than 30 species of birds. To date, the species infecting birds are C. baileyi, C. galli and C. meleagridis. In this study, the morphological, clinical and molecular characteristics of a Brazilian ostrich isolate of Cryptosporidium are described. The oocysts of this Brazilian isolate are larger and more elongated than those of Cryptosporidium previously reported in ostriches, which were morphologically similar to C. meleagridis. Morphological, biological and molecular analyses demonstrated similarity of this ostrich isolate with C. baileyi, suggesting that there are at least two Cryptosporidium species infecting ostriches; one with molecular, biological and morphological characteristics related to C.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clinical signs; Cryptosporidium; Morphology; Ostriches; PCR-RFLP. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000200008 |
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| Almeida,Álvaro M. R.; Piuga,Fernanda F.; Marin,Silvana R. R.; Binneck,Eliseu; Sartori,Fábio; Costamilan,Leila M.; Teixeira,Maria R. O.; Lopes,Marcelo. |
| Cercospora kikuchii, involved with the defoliation of soybean (Glycine max) plants, is normally associated with Septoria glycines in late season. Seventy-two isolates from different regions in Brazil, obtained mainly from purple stained seeds, showed phenotypic variation. Cercosporin content and rate of colony growth was higly variable among isolates. A strong correlation between cercosporin content and virulence was identified. Genetic variation among and within populations was evaluated based on 86 RAPD loci. The RAPD analysis clustered all isolates into seven groups. No relationship was observed between RAPD groups and geographic origin or cercosporin content. The sequence of the internal spacer regions (ITS1-5.8S-ITS2) from 13 isolates chosen according... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotypic diversity; PCR-RFLP; RAPD; ITS sequence; Virulence. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000600005 |
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| Machado,M.A.; Nascimento,C.S.; Martinez,M.L.; Silva,M.V.G.B.; Campos,A.L.; Teodoro,R.L.; Verneque,R.S.; Guimarães,S.E.F.. |
| A associação entre os alelos do loco BoLA-DRB3.2, identificados pela técnica de PCR-RFLP, e a produção de leite na raça Gir foi estudada por meio da análise de dados moleculares e fenotípicos de 424 vacas Gir, utilizando um modelo misto, sob modelo animal. Os dados moleculares consistiam dos genótipos dos animais para os alelos do loco BoLA-DRB3.2 e os dados fenotípicos eram referentes à produção de leite em até 305 dias de lactação. O loco é altamente polimórfico nesta raça, sendo identificados sete alelos (BoLA-DRB3.2*4, *8, *11, *19, *28, *41 e *48) que não haviam sido encontrados em animais zebuínos. Dois alelos (*16 e *29) estavam significativamente associados com maiores produções de leite, sugerindo que o próprio loco BoLA-DRB3.2 ou um QTL a ele... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BoLA-DRB3; PCR-RFLP; Gene candidato; Bovinos; Gir. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000300017 |
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| Registros recuperados: 66 | |
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